Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC
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چکیده
منابع مشابه
Functional analysis of minichromosome replication: bidirectional and unidirectional replication from the Escherichia coli replication origin, oriC.
Replicating molecules of minichromosomes pCM959 and pOC24 were analyzed by electron microscopy. Replication of pCM959 proceeded bidirectionally from the replication origin, oriC, in about 60% of the molecules; the rest of the molecules replicated unidirectionally in either direction. pOC24, in which deoxyribonucleic acid to the right (clockwise) of the oriC segment is deleted, seemed to replica...
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The formation of nucleoprotein complexes between the Escherichia coli initiator protein DnaA and the replication origin oriC was analysed in vitro by band-shift assays and electron microscopy. DnaA protein binds equally well to linear and supercoiled oriC substrates as revealed by analysis of the binding preference to individual DnaA boxes (9-mer repeats) in oriC, and by a competition band-shif...
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An enzyme system that replicates plasmids bearing the origin of the Escherichia coli chromosomes (oriC) has the following physiologically relevant features. The system (i) depends completely on low levels of exogenously furnished supercoiled oriC plasmids, (ii) uses only those plasmids that contain the intact oriC region of about 245 base pairs, (iii) initiates replication within or near the or...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1980
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.143.2.1049-1053.1980